Tragopogon mirus is a recently formed allotetraploid species, with Tragopogon dubius and Tragopogon porrifolius as the parental species. A study was conducted on Tragopogon populations in Oregon, USA to determine if T. dubius and T. porrifolius hybridization and subsequent polyploid speciation had occurred. We examined ploidy levels as well as nucleotide data from the chloroplast trnT-trnL spacer and nuclear ribosomal ITS regions. While no hybridization or polyploid speciation was found, a seven base pair length mutation found in the chloroplast trnT-trnL spacer region should prove useful for determining parentage in populations of T. mirus.